Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis

Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis

Blog Article

Abstract The discovery and characterization of antigen-specific CD8+ Lunchbox T cell clonotypes typically involves the labor-intensive synthesis and construction of peptide-MHC tetramers.We adapt single-chain trimer (SCT) technologies into a high throughput platform for pMHC library generation, showing that hundreds can be rapidly prepared across multiple Class I HLA alleles.We use this platform to explore the impact of peptide and SCT template mutations on protein expression yield, thermal stability, and functionality.SCT libraries were an efficient tool for identifying T cells recognizing commonly reported viral epitopes.

We then Rear Camera Kit construct SCT libraries to capture SARS-CoV-2 specific CD8+ T cells from COVID-19 participants and healthy donors.The immunogenicity of these epitopes is validated by functional assays of T cells with cloned TCRs captured using SCT libraries.These technologies should enable the rapid analyses of peptide-based T cell responses across several contexts, including autoimmunity, cancer, or infectious disease.